Erős-Honti, Zs., Kovács, M. G., Szedlay, Gy., Jakucs, E. (2008): Morphological and molecular characterization of Humaria and Genea ectomycorrhizae from Hungarian deciduous forests. Mycorrhiza 18: 133-143.

Nyomtatóbarát változatPDF version
Teljes hivatkozás: 
Erős-Honti, Zs., Kovács, M. G., Szedlay, Gy., Jakucs, E. (2008): Morphological and molecular characterization of Humaria and Genea ectomycorrhizae from Hungarian deciduous forests. Mycorrhiza 18: 133-143.
Rövid hivatkozás: 
Erős-Honti et al. (2008)
Első szerző: 
Erős-Honti Zsolt
Év: 
2008
Összefoglalás: 

Morphological and molecular characterization of Humaria and Genea ectomycorrhizae from Hungarian deciduous forests
Zsolt Erős-Honti, Gábor M. Kovács, Gyöngyi Szedlay, Erzsébet Jakucs

The ectomycorrhizae (EM) of Humaria and Genea, two closely related genera of the Pyronemataceae (Ascomycetes), were regularly found in different deciduous forests of Hungary. In the present paper, the morphology and anatomy of these EM are described in detail, including morphometric analyses. Identification of the EM was carried out by molecular taxonomic analyses of the nrDNA ITS seqences obtained from mycorrhizae, herbarium ascomata, and public databases. The anatomy of the EM, examined during this work, was almost identical. They possessed angular outer and epidermoid inner mantle layers and warted, thick-walled emanating hyphae. Ten of our EM sequences grouped into the clade of Humaria hemisphaerica sequences and one into the genus Genea. Both molecular taxonomic analysis and morphometry differentiated three sub-groups within the clade of Humaria, and these methods also clearly separated the EM of Genea from those of Humaria. We may suppose that the previous morphological-anatomical descriptions, lacking molecular taxonomic identification, do not concern the denominated taxa. As a consequence, we stress the importance of revaluating the literature data, based on morphotyping of Humaria and Genea EM, to prevent misidentification in future studies. The presented work demonstrates that combining molecular and morphological analysis is essential for the unambiguous identification of the EM formed by problematic taxa.

Megjegyzések: 

Morphological and molecular characterization of Humaria and Genea ectomycorrhizae from Hungarian deciduous forests
Zsolt Erős-Honti, Gábor M. Kovács, Gyöngyi Szedlay, Erzsébet Jakucs

Tartalom:
Introduction
Materials and methods
Sampling sites
Sampling
Characterization of EM morphology and anatomy
Study design and statistical analysis in morphometry
Herbarium samples
Molecular analysis
DNA extraction
PCR and sequencing
Phylogenetic analyses
Results
Common morphological-anatomical features of the EM
Phylogenetic inference
Morphometric analysis
Discussion
Acknowledgements
Appendix
References

Ectomycorrhiza, Genea, Humaria, Anatomy, Morphometry, Phylogenetic analysis

Kiadó: 
Springer-Verlag
Folyóirat: 
Mycorrhiza
Lelőhely: 
ER Archívum (2008/P-032)
Típus: 
tudományos publikáció, konferencia szakcikk (teljes)
Erdőrezervátumok: 
Katalógusba vette: 
Gulyás Györgyi
Katalógusbavétel időpontja: 
2011-05-09